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How to get great results with correctly crimped vials?


A correctly crimped vial is neither over-crimped nor under-crimped.

  • Over-crimping may cause coring or poor septum resealing
  • Under-crimping can cause evaporation problems.

With proper adjustment of the vial crimpers, perfect crimping can be achieved every time. When the vial is crimped perfectly, the cap should not be able to rotate and the septum should appear smooth and level.

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GC/HPLC Vial Septa Selection Guide


Optimise performance and results by choosing the right septum for your application:

PTFE Septa

  • Exceptional solvent resistance
  • Only suitable for single injection use – not recommended for sample storage before or after injection
  • Not resealable
  • The most economical septa
  • Maximum service temperature 260°C

PTFE/Silicone Septa

  • Excellent resealing capabilities – highly recommended for multiple injections and sample storage
  • Autoclavable and excellent resistance to coring
  • PTFE chemical resistance until pierced then the septa will have the compatibility of silicone
  • Temperature range -40°C to 200°C

Pre-slit PTFE/Silicone Septa

  • Reduces the possibility of coring with blunt tipped needles or for applications using a thin gauge needle
  • Used to prevent vacuum from forming inside the vial
  • Temperature range -40°C to 200°C

PTFE/Silicone/PTFE Septa

  • Recommended for multiple injections due to above average resealing capabilities
  • Autoclavable and excellent resistance to coring
  • Recommended for demanding applications such as internal standards, trace analysis or applications where there will be a long time between injections
  • Temperature range -40°C to 200°C

PTFE/Red Rubber Septa

  • The most economical septa used in routine analysis
  • Easy to pierce with moderate resealability
  • PTFE chemical resistance until pierced then the septa will have the compatibility of rubber
  • Not recommended when retaining samples for further analysis or for multiple injections
  • Temperature range -40°C to 110°C

Moulded Polypropylene Septa

  • Only suitable for single injection use – not recommended for sample storage before or after injection
  • Not resealable
  • Temperature range 0°C to 130°C

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Can glass vials be frozen and what do you need to know?


Most glass vials can tolerate temperatures down to -4˚C, a common temperature for short-term storage of test samples without requiring special precautions. A glass vial containing mostly organic solvents can be stored at temperatures as low as -80˚C. However, extra care should be taken with vials and closures made from polypropylene since these materials become increasingly brittle at temperatures below 0˚C. Here are some general guidelines to follow when freezing vials:

Storing Vials:

When storing vials, it is important that the vials avoid intense direct sunlight. The best results are obtained when the samples are kept in a plastic vial rack during storage and thawing.

Many liquids expand when frozen and this is particularly true when the sample is mostly water. When frozen water expands, it exerts considerable pressures on closed containers and may cause them to burst. Therefore, any vial or other container that will be stored below the freezing point of water (0˚C) should not be filled to the rim in order to allow adequate space for expansion.

For extreme low temperatures, snap caps are not recommended. The best low temperature vials should have large internal volumes and relatively small openings such as clear glass 8-425 screw thread vials or clear glass standard opening crimp top vials. It is possible to use large opening vials with good results, but handling precautions must be strictly followed including the use of a closed top cap or a bonded septum open top cap.

When storing vials for extended periods of time or at very low temperatures, a crimp seal or solid top screw thread cap is recommended.

Thawing Frozen Vials

Cold and frozen vials must be handled with extreme care. In general, glass vials will crack when exposed to rapid temperature changes (particularly for amber vials). Do not place cold vials directly on warm surfaces. Physical shock must be avoided. Sitting a cold vial on a metal surface greatly increases the potential for base breakage.

Be aware that very cold vials have a tendency to stick to any surface until completely thawed. Additionally, it is best to avoid disturbing vials until all traces of frozen liquid are gone and the contents have reached ambient temperature.

Warming Frozen Vials:

Vials should be warmed gradually. It is very important to avoid rapid changes in temperature especially when warming the vial. It is not advisable to use water baths, heat guns, ovens, or other elevated heat sources to warm vials. Rather, it is generally best to thaw frozen vials by allowing them to come to room temperature slowly while sitting on a non-heat conductive surface at ambient temperatures. Vials stored at temperatures below -20°C should be allowed to equilibrate in a refrigerator (-4°C to 0°C) before finally being brought to room temperature. This two-stage process minimizes the possibility of stress fractures.

Considerations for PTFE/Silicone Septa:

Many materials used for septa have a lower temperature limit of about -40˚C. PTFE/ Silicone septa will maintain an adequate seal at temperatures down to -80˚C, but care must be taken to avoid over-tightening closures. There should be no noticeable deformation of the septum when properly tightened. Please note that we publish a lower usable temperature of -40˚C for PTFE/Silicone septa. This limit refers to the temperature where the freezing point of water (0˚C) should not be filled to the rim in order to allow adequate space for expansion.

For extreme low temperatures, snap caps are not recommended. The best low temperature vials should have large internal volumes and relatively small openings such as clear glass 8-425 screw thread vials or clear glass standard opening crimp top vials. It is possible to use large opening vials with good results, but handling precautions must be strictly followed including the use of a closed top cap or a bonded septum open top cap.

When storing vials for extended periods of time or at very low temperatures, a crimp seal or solid top screw thread cap is recommended.

Thawing Frozen Vials

Cold and frozen vials must be handled with extreme care. In general, glass vials will crack when exposed to rapid temperature changes (particularly for amber vials). Do not place cold vials directly on warm surfaces. Physical shock must be avoided. Sitting a cold vial on a metal surface greatly increases the potential for base breakage.

Be aware that very cold vials have a tendency to stick to any surface until completely thawed. Additionally, it is best to avoid disturbing vials until all traces of frozen liquid are gone and the contents have reached ambient temperature.

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First Vial is a supplier of an ever-expanding line of chromatography consumable products. We serve customers in laboratories around the world.  read more ...

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